LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2237..2333) /label=attL2 /note="attL2 (c)" misc_signal complement(2348..2367) /note="T7 Promoter/priming site (c)" primer_bind 2375..2391 /note="M13 Reverse priming site" gene 2504..3313 /note="Kanamycin resistance gene" rep_origin 3434..4107 /note="pUC origin" vector join(2245..4110,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..2230 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:39891 IMAGE:4478643" /tissue_type="Testis, embryonal carcinoma" /clone_lib="NIH_MGC_92" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 692..2230 /gene="GALNT1" /gene_synonym=GALNAC-T1 /db_xref="GeneID:2589" /db_xref="HGNC:4123" /db_xref="MIM:602273" CDS 734..2230 /dnas_title="GALNT1 protein" /gene="GALNT1" /gene_synonym=GALNAC-T1 /codon_start=1 /product="GALNT1 protein" /protein_id="AAH38440.1" /db_xref="GI:116284114" /db_xref="GeneID:2589" /db_xref="HGNC:4123" /db_xref="MIM:602273" /translation="MGKPVVIPKEDQEKMKEMFKINQFNLMASEMIALNRSLPDVRLE GCKTKVYPDNLPTTSVVIVFHNEAWSTLLRTVHSVINRSPRHMIEEIVLVDDASERDF LKRPLESYVKKLKVPVHVIRMEQRSGLIRARLKGAAVSKGQVITFLDAHCECTVGWLE PLLARIKHDRRTVVCPIIDVISDDTFEYMAGSDMTYGGFNWKLNFRWYPVPQREMDRR KGDRTLPVRTPTMAGGLFSIDIDYFQEIGTYDAGMDIWGGENLEISFRIWQCGGTLEI VTCSHVGHVFRKATPYTFPGGTGQIINKNNRRLAEVWMDEFKNFFYIISPGVTKVDYG DISSRVGLRHKLQCKPFSWYLENIYPDSQIPRHYFSLGEIRNVETNQCLDNMARKENE KVGIFNCHGMGGNQVFSYTANKEIRTDDLCLDVSKLNGPVTMLKCHHLKGNQLWEYDP VKLTLQHVNSNQCLDKATEEDSQVPSIRDCNGSRSQQWLLRNVTLPEIF" misc_feature 692..2230 /note="GALNT1 coding region" misc_difference 1452..1452 /gene="GALNT1" /gene_synonym=GALNAC-T1 /note="'T' in cDNA is 'G' in the human genome; amino acid difference: 'I' in cDNA, 'R' in the human genome." ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cgttctagag ccagtacaaa agcctcatga 721 aggtcctgga gaaatgggga aaccagtcgt cattcctaaa gaggatcaag aaaagatgaa 781 agagatgttt aaaatcaatc agttcaattt aatggcaagt gagatgattg cactcaacag 841 atctttacca gatgttaggt tagaagggtg taaaacaaag gtgtatccag ataatcttcc 901 tacaacaagt gtggtgattg ttttccacaa tgaggcttgg agcacacttc tgcgaactgt 961 ccatagtgtc attaatcgct caccaagaca catgatagaa gaaattgttc tagtagatga 1021 tgccagtgaa agagactttt tgaaaaggcc tttagagagt tatgtgaaaa aactaaaagt 1081 accagttcat gtaattcgaa tggaacaacg ttctggattg atcagagcta gattaaaagg 1141 agctgctgtg tctaaaggcc aagtgatcac cttcctggat gcccattgtg agtgtacagt 1201 gggatggctg gagcctctct tggccaggat caaacatgac aggagaacag tggtgtgtcc 1261 catcatcgat gtgatcagtg atgatacttt tgagtacatg gcaggctctg atatgaccta 1321 tggtgggttc aactggaagc tcaattttcg ctggtatcct gttccccaaa gagaaatgga 1381 cagaaggaaa ggtgatcgga ctcttcctgt caggacacct accatggcag gaggcctttt 1441 ttcaatagac atagattact ttcaggaaat tggaacatat gatgctggaa tggatatttg 1501 gggaggagaa aacctagaaa tttcctttag gatttggcag tgtggaggaa ctttggaaat 1561 tgttacatgc tcacatgttg gacatgtgtt tcggaaagct acaccttaca cgtttccagg 1621 aggcacaggg cagattatca ataaaaataa cagacgactt gcagaagtgt ggatggatga 1681 attcaagaat ttcttctata taatttctcc aggtgttaca aaggtagatt atggagatat 1741 atcgtcaaga gttggtctaa gacacaaact acaatgcaaa cctttttcct ggtacctaga 1801 gaatatatat cctgattctc aaattccacg tcactatttc tcattgggag agatacgaaa 1861 tgtggaaacg aatcagtgtc tagataacat ggctagaaaa gagaatgaaa aagttggaat 1921 ttttaattgc catggtatgg ggggtaatca ggttttctct tatactgcca acaaagaaat 1981 tagaacagat gacctttgct tggatgtttc caaacttaat ggcccagtta caatgctcaa 2041 atgccaccac ctaaaaggca accaactctg ggagtatgac ccagtgaaat taaccctgca 2101 gcatgtgaac agtaatcagt gcctggataa agccacagaa gaggatagcc aggtgcccag 2161 cattagagac tgcaatggaa gtcggtccca gcagtggctt cttcgaaacg tcaccctgcc 2221 agaaatattc TAGGACCCAG CTTTCTTGTA CAAAGTTGGC ATTATAAGAA AGCATTGCTT 2281 ATCAATTTGT TGCAACGAAC AGGTCACTAT CAGTCAAAAT AAAATCATTA TTTGCCATCC 2341 AGCTGATATC CCCTATAGTG AGTCGTATTA CATGGTCATA GCTGTTTCCT GGCAGCTCTG 2401 GCCCGTGTCT CAAAATCTCT GATGTTACAT TGCACAAGAT AAAATAATAT CATCATGAAC 2461 AATAAAACTG TCTGCTTACA TAAACAGTAA TACAAGGGGT GTTATGAGCC ATATTCAACG 2521 GGAAACGTCG AGGCCGCGAT TAAATTCCAA CATGGATGCT GATTTATATG GGTATAAATG 2581 GGCTCGCGAT AATGTCGGGC AATCAGGTGC GACAATCTAT CGCTTGTATG GGAAGCCCGA 2641 TGCGCCAGAG TTGTTTCTGA AACATGGCAA AGGTAGCGTT GCCAATGATG TTACAGATGA 2701 GATGGTCAGA CTAAACTGGC TGACGGAATT TATGCCTCTT CCGACCATCA AGCATTTTAT 2761 CCGTACTCCT GATGATGCAT GGTTACTCAC CACTGCGATC CCCGGAAAAA CAGCATTCCA 2821 GGTATTAGAA GAATATCCTG ATTCAGGTGA AAATATTGTT GATGCGCTGG CAGTGTTCCT 2881 GCGCCGGTTG CATTCGATTC CTGTTTGTAA TTGTCCTTTT AACAGCGATC GCGTATTTCG 2941 TCTCGCTCAG GCGCAATCAC GAATGAATAA CGGTTTGGTT GATGCGAGTG ATTTTGATGA 3001 CGAGCGTAAT GGCTGGCCTG TTGAACAAGT CTGGAAAGAA ATGCATAAAC TTTTGCCATT 3061 CTCACCGGAT TCAGTCGTCA CTCATGGTGA TTTCTCACTT GATAACCTTA TTTTTGACGA 3121 GGGGAAATTA ATAGGTTGTA TTGATGTTGG ACGAGTCGGA ATCGCAGACC GATACCAGGA 3181 TCTTGCCATC CTATGGAACT GCCTCGGTGA GTTTTCTCCT TCATTACAGA AACGGCTTTT 3241 TCAAAAATAT GGTATTGATA ATCCTGATAT GAATAAATTG CAGTTTCATT TGATGCTCGA 3301 TGAGTTTTTC TAATCAGAAT TGGTTAATTG GTTGTAACAC TGGCAGAGCA TTACGCTGAC 3361 TTGACGGGAC GGCGCAAGCT CATGACCAAA ATCCCTTAAC GTGAGTTACG CGTCGTTCCA 3421 CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG 3481 CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA 3541 TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA 3601 TACTGTTCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC 3661 TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG 3721 TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC 3781 GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT 3841 ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC 3901 GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG 3961 GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG 4021 CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT 4081 GGCCTTTTGC TGGCCTTTTG CTCACATGTT //